Pdf immunophenotyping of classical hodgkins lymphoma by flow. Hodgkin lymphoma diagnosis leukemia and lymphoma society. Leukemialymphoma immunophenotyping profile labcorp. Flow cytometric analysis of cytologic specimens in. Flow cytometry immunophenotyping may be performed on blood, bone marrow, or other samples to provide this additional information. The current who classification uses morphologic, immunophenotypic, genotypic, and. Cellular immunophenotyping, characterizing cells by using antibodies directed against cell surface markers, is generally regarded as a fundamental element in establishing a diagnosis of tissue involvement by hematolymphoid malignancies, when used in conjunction with morphologic assessment. Express surface immunoglobulin, immunoglobulin m, or immunoglobulin d are characteristically cd5. This process is used in basic research and to help diagnose diseases, such as specific types of leukemia and lymphoma.
Flow cytometric immunophenotyping fci is recommended in the evaluation of cerebrospinal fluid csf specimens for hematologic neoplasms. Advances in immunophenotyping which include development of new antibodies and new protocols for retrievingunmasking antigens in routinely fixed and processed surgical biopsy material together with the wide acceptance of modern lymphoma classification systems has made confident and constant subclassification of lymphomas possible. With the exception of rare cases with dramatically increased malignant populations, isolation of pure viable tumor cells has. These markers are usually functional membrane proteins involved in cell communication, adhesion, or metabolism. The diagnosis of chl currently relies on a combination of morphologic findings and immunohistochemical stains. Aug 23, 2018 immunophenotyping is a test used to identify cells on the basis of the types of markers or antigens present on the cells surface, nucleus, or cytoplasm. Sample cases click here for instructions on how to download the free fcs express reader to view and manipulate the sample cases.
Immunophenotyping of classical hodgkins lymphoma by flow. To evaluate the role of flow cytometric immunophenotyping fci in diagnosis and characterization of lymphoma tissue specimens from egyptian patients. Review article flow cytometric immunophenotyping for hematologic neoplasms fiona e. The aim of this study was to adapt the immunohistochemical protocol used for immunophenotyping of canine lymphoma to the personal flow cytometer for rapid, effective. In a retrospective analysis the authors studied the relation between the immunologic phenotype of bcell nonhodgkins lymphoma nhl and disease free survival. Some of these tests may be repeated both during and after therapy to measure the effects of treatment. Immunophenotyping using flow cytometry has become the method of choice in identifying and sorting cells within complex populations, for example the analysis of immune cells in a blood sample. In our experience, while the neoplastic population the hodgkins reedsternberg cell of classical hodgkins lymphoma chl cannot be detected by flow cytometry assays designed to detect b and t cell non hodgkins. Guidelinesforthemanagementof non2hodgkinsandhodgkins. Lack of surface immunoglobulin light chain expression by flow cytometric immunophenotyping can help diagnose peripheral bcell lymphoma shiyong li, md, phd, james r.
The diagnosis of classical hodgkin lymphoma chl has been made in tissue sections. Hanson and bertram schnitzer department of pathology, university of michigan medical school, ann arbor, michigan cytologic evaluation of body fluids and fine needle aspirations fna is frequently required in patients with hematologic dis eases. It provides a lot of useful information in this setting that transfers directly from laboratory to clinical management of patients. Hanson and bertram schnitzer department of pathology, university of michigan medical school, ann arbor. Flow cytometry immunophenotypic diagnosis of bcell nonhodgkin. In a retrospective analysis the authors studied the relation between the immunologic phenotype of bcell nonhodgkins lymphoma nhl and diseasefree survival. Abstract although the diagnosis of classical hodgkin lymphoma chl is traditionally made morphologically in tissue sections, it is now.
If both tissue flow cytometry and histology are required, submit one portion of fresh specimen in transport medium or saline for flow. O immunophenotyping, a process that allows the hematopathologist to study the. Flow cytometric detection of the classical hodgkin lymphoma. Immunophenotyping in the differential diagnosis of. Flow cytometry can diagnose classical hodgkin lymphoma in. A minor percentage immunophenotyping of lymphoma patients can be achieved through a variety of tests including. Pdf lymphomas constitute approximately 5% of all malignant neoplasms of the head and neck. Oct 26, 2012 in our experience, while the neoplastic population the hodgkins reedsternberg cell of classical hodgkins lymphoma chl cannot be detected by flow cytometry assays designed to detect b and t cell non hodgkins lymphoma, six and ninecolor reagent combinations are available to detect these cells with high diagnostic sensitivity and specificity.
These tests may suggest lymphoma or leukemia, but more information is generally needed to confirm a diagnosis and to identify a specific type of leukemia or lymphoma. Immunophenotyping of nonhodgkins lymphomas in sudan. Immunophenotyping by flow cytometry lab tests online. Immunophenotyping of murine precursor bcell leukemia. Cellular immunophenotyping, characterizing cells by using antibodies directed against cell surface markers, is generally regarded as a fundamental element in establishing a diagnosis of. When cll presents as predominant lymphadenopathy, follicular lymphoma and. Tissue flow cytometry immunophenotyping in the diagnosis and classification of non. The hematopathologist looks for the presence of certain antigens or markers on the surface of the cells in order to identify hodgkin and rs cells and confirm a diagnosis of hl.
Ki67 overexpression and patient overall or event free survival. Flow cytometric detection of the classical hodgkin. The role of immunophenotyping in differential diagnosis of. The name often refers to just the cancerous versions rather than all such tumors. Flow cytometric immunophenotyping for hematologic neoplasms.
Flow cytometric analysis of cytologic specimens in hematologic disease curtis a. Apr 30, 2020 on immunophenotyping, tumor cells in mcl are monoclonal b cells with the following characteristics. The aim of this study was to adapt the immunohistochemical protocol used for immunophenotyping of canine lymphoma to the personal flow cytometer for rapid, effective and userfriendly application to the diagnosis and prognosis of canine lymphoma and to demonstrate its practicality for widespread veterinary application. Pdf more than two decades have past since the recognition of nonhodgkins lymphomas nhls as neoplasms of the. Immunophenotyping improved the diagnostic accuracy by 10% to 45% for a number. Pdf update on classification of lymphomas researchgate. Pdf anaplastic large cell lymphoma alcl is usually diagnosed by histologic and immunohistochemical analysis. Immunophenotypic analysis identifies specific markers of lineage b or. Lack of surface immunoglobulin light chain expression by flow. Immunophenotyping is the use of antibodies against cell surface receptors to differentiate between normal and abnormal tissues for a variety of human diseases, including lymphoma, leukemia, and aids. Diffuse large bcell lymphoma dlbcl forms a heterogeneous collection of aggressive nonhodgkins lymphoma in which three principle classes of neoplasia have been defined according to gene expression and immunophenotyping studies. A biopsy of suspected lymphoma tissue typically a lymph node, and subsequent evaluation by a trained pathologist is the only way to definitively diagnose lymphoma.
Tissue flow cytometry immunophenotyping in the diagnosis and. Advances in immunophenotyping which include development of new antibodies and new protocols for retrievingunmasking antigens in routinely fixed and processed surgical biopsy. Leukemia and lymphoma may also present with blasts and abnormal cells in the blood or body fluids and immunophenotyping again can be performed to identify immature or. Fci using 2 and 3 color staining approaches, was performed on 50 fresh lymph nodes specimen from cairo nci patients with suspected lymphoma presenting with either localized or generalized lymphadenopathy. Stacchini a, aliberti s, chiusa l, chiarle r, novero d. Express surface immunoglobulin, immunoglobulin m, or. This book offers complete coverage of this topic, including all basic and clinical aspects. Correlation with relapsefree survival article pdf available in american journal of pathology 11. Immunophenotypic variations in mantle cell lymphoma. This technique allows rapid and easy phenotyping of each cell line in a heterogeneous sample according to the presence or absence of a protein combination.
A clinical study ashish gupta1, abhijit pal1, silas supragya nelson2 1assistant professor, department of medicine, nscb medical college, jabalpur, madhya pradesh, india, 2associate professor, department of medicine, nscb medical college, jabalpur, madhya pradesh, india meaning white blood. A clinical study ashish gupta1, abhijit pal1, silas supragya nelson2 1assistant professor, department of medicine, nscb medical college. Flow cytometric analysis of cd123 is useful for immunophenotyping. Bcell lymphomas arise from different stages of differentiation of bcells and constitute a broad. Pdf immunophenotyping of acute leukemias from biology. Immunophenotyping is a very common flow cytometry test in which fluorophoreconjugated antibodies are used as probes for staining target cells with high avidity and affinity. Pdf flow cytometric immunophenotyping of nonhodgkins. Pdf flow cytometric immunophenotyping of anaplastic. Nci dictionary of cancer terms national cancer institute. Pdf immunophenotyping of acute leukemias from biology to.
Immunophenotyping is an essential part of the modern diagnostic workup of acute leu. They found only 25% cellular viability within 11 cases of high. Built on the research and validation work of the euroflow consortium on the characterization of. Immunophenotyping using flow cytometry would be the main method for differentiating these disorders. Diffuse large bcell lymphoma dlbcl the single largest category of lymphoma, is a heterogeneous group of malignant lymphomas with several morphologic features. Histopathology, morphology and immunophenotyping of cll. The hematopathologist may use a lab test called immunophenotyping to distinguish hodgkin lymphoma from other types of lymphoma or other cancerous or noncancerous conditions. Correlation between flow cytometry and histologic findings. Lymphadenopathy 19 20 or swelling of lymph nodes, is the primary presentation in lymphoma. The role of immunophenotyping in differential diagnosis of chronic lymphocytic leukemia methods patients we analyzed 211 consecutive untreated patients. What is the role of immunophenotypic analysis in the workup of non.
What are the immunophenotyping characteristics of mantle. Immunophenotyping is an essential part of the modern diagnostic workup of acute leukemias and thus for an appropriate treatment of these complex and heterogeneous. Immunophenotyping of diffuse large bcell lymphoma dlbcl. Foon2 1division of hematopathology, department of pathology, and 2division of hematologyoncology, department of medicine, university of pittsburgh school of medicine, pa flow cytometric immunophenotyping re. Article information, pdf download for immunophenotyping of murine. Lymphoma is a group of blood cancers that develop from lymphocytes a type of white blood cell. Ki67 over expression and patient overall or event free survival. Traditionally, 23 of dogs with lymphoma are classified as b cell and are t cell.
Immunophenotyping is the use of antibodies against cell surface receptors to differentiate between normal and abnormal tissues for a variety of human diseases, including lymphoma. On immunophenotyping, tumor cells in mcl are monoclonal b cells with the following characteristics. In veterinary medicine, currently canine lymphoma is broken down into two main immunophenotypes, b and t cell. Classical hodgkin lymphoma chl is a relatively uncommon b cellderived neoplasm that presents with rare malignant cells in an abundant reactive background. Lineage definition is the first goal leading to proper initial therapy. Diffuse large bcell lymphoma dlbcl forms a heterogeneous collection of aggressive nonhodgkins lymphoma in which three principle classes of neoplasia have been defined. Immunophenotyping may also be used to separate cells into different groups based on the markers they have on the surface. Immunophenotyping is an essential part of the modern diagnostic workup of acute leukemias and thus for an appropriate treatment of these complex and heterogeneous diseases. Pdf flow cytometric immunophenotyping of cerebrospinal. Tissue flow cytometry immunophenotyping in the diagnosis and classification of.
Pdf on oct 26, 2012, jonathan r fromm and others published. Immunophenotypic analysis of lymph node, bone marrow, peripheral. The presence of necrosis often leads to underrepresentation of malignant cells within a sample specimen, as demonstrated by zander et al. Built on the research and validation work of the euroflow consortium on the characterization of hematological malignancies for improved diagnostic outcomes, the bd oneflow solution brings the standardization of leukemia and lymphoma immunophenotyping on e step forward.
Flow cytometric immunophenotyping of nonhodgkins lymphomas and related disorder. Lack of surface immunoglobulin light chain expression by. The hematopathologist may use a lab test called immunophenotyping to distinguish hodgkin lymphoma from other types of lymphoma or other cancerous or noncancerous conditions based on the antigens or markers on the surface of the cells. Demurtas a, stacchini a, aliberti s, chiusa l, chiarle r, novero d. Rapid, effective and userfriendly immunophenotyping of. Immunophenotyping leukemias and lymphomas click here to start. What are the immunophenotyping characteristics of mantle cell. Tissue flow cytometry immunophenotyping in the diagnosis.